Stimulated Raman Scattering Microscopy for Label-Free Chemical Imaging
نویسندگان
چکیده
منابع مشابه
Correction for Lu et al., Label-free DNA imaging in vivo with stimulated Raman scattering microscopy.
Label-free DNA imaging is highly desirable in biology and medicine to perform live imaging without affecting cell function and to obtain instant histological tissue examination during surgical procedures. Here we show a label-free DNA imaging method with stimulated Raman scattering (SRS) microscopy for visualization of the cell nuclei in live animals and intact fresh human tissues with subcellu...
متن کاملQuantitative chemical imaging with multiplex stimulated Raman scattering microscopy.
Stimulated Raman scattering (SRS) microscopy is a newly developed label-free chemical imaging technique that overcomes the speed limitation of confocal Raman microscopy while avoiding the nonresonant background problem of coherent anti-Stokes Raman scattering (CARS) microscopy. Previous demonstrations have been limited to single Raman band measurements. We present a novel modulation multiplexin...
متن کاملLabel-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy.
The recently developed Coherent Anti-stokes Raman Scattering (CARS) microscopy and Stimulated Raman Scattering (SRS) microscopy have provided new methods to visualize the localization and regulation of biological molecules without the use of invasive and potentially perturbative labels. They allow rapid imaging of specific molecules with high resolution and sensitivity. These tools have been ef...
متن کاملLabel-free DNA imaging in vivo with stimulated Raman scattering microscopy
MEDICAL SCIENCES, APPLIED PHYSICAL SCIENCES Correction for “Label-free DNA imaging in vivo with stimulated Raman scattering microscopy,” by Fa-Ke Lu, Srinjan Basu, Vivien Igras, Mai P. Hoang, Minbiao Ji, Dan Fu, Gary R. Holtom, Victor A. Neel, Christian W. Freudiger, David E. Fisher, and X. Sunney Xie, which appeared in issue 37, September 15, 2015, of Proc Natl Acad Sci USA (112:11624–11629; f...
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Label-free chemical contrast is highly desirable in biomedical imaging. Spontaneous Raman microscopy provides specific vibrational signatures of chemical bonds, but is often hindered by low sensitivity. Here we report a three-dimensional multiphoton vibrational imaging technique based on stimulated Raman scattering (SRS). The sensitivity of SRS imaging is significantly greater than that of spon...
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ژورنال
عنوان ژورنال: Optics and Photonics News
سال: 2009
ISSN: 1047-6938,1541-3721
DOI: 10.1364/opn.20.12.000030